Toxicology and Industrial Health - ۲۰۱۴

Research has suggested that natural antioxidant, crocin, an active ingredient of saffron, may protect against diazinon (DZN)-induced toxicity. Although increased production of lipid peroxidation by DZN in rat aorta has been shown previously, the effects of DZN on oxidative stress-induced apoptosis in vascular system have not been evaluated. In this study, the effect of crocin on DZN-induced apoptosis in rat vascular system was investigated. The rats were divided into ۷ groups: corn oil (control), DZN (۱۵ mg/kg/day, gavage), crocin (۱۲.۵, ۲۵, and ۵۰ mg/kg/day, intraperitoneally (i.p.)) + DZN, vitamin E (۲۰۰ IU/kg, i.p., ۳ days a week) + DZN, and crocin (۵۰ mg/kg/day, i.p.). The treatments were continued for ۴ weeks. Levels of apoptotic (Bax, caspase ۳, and caspase ۹) and antiapoptotic proteins (Bcl۲) were analyzed by Western blotting. Transcript levels of Bax and Bcl۲ genes were determined using quantitative real-time polymerase chain reaction. Results showed DZN-induced apoptosis by activation of caspase ۹ and caspase ۳ and by increasing the Bax/Bcl۲ ratio (both protein and messenger RNA levels). Crocin and vitamin E inhibited apoptosis induced by DZN. In summary, subchronic exposure to DZN induced caspase-mediated apoptosis, and crocin reduced the toxic effects of DZN by inhibiting apoptosis in aortic tissue.